| 质粒名称: | pCMV6 BDNF |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 39857 |
| 存储实验室: | Yves-Alain Barde |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV6-XL3 |
| 载体出品公司: | Origene |
| 空载体大小: | 4700bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BDNF |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 750bp |
| GenBank编号 | X55573 |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI(未破坏) |
| 3’克隆位点: | XbaI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | T3 |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV6 BDNF was a gift from Yves-Alain Barde (Addgene plasmid # 39857 ; http://n2t.net/addgene:39857 ; RRID:Addgene_39857) |
| 参考文献部分: | Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain. Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde YA. EMBO J. 1990 Aug;9(8):2459-64. PubMed 2369898 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV6 BDNF 5385 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV6 BDNF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5385)
AUTHORS Hofer M, Pagliusi SR, Hohn A, Leibrock J, Barde YA
TITLE Regional distribution of brain-derived neurotrophic factor mRNA in
the adult mouse brain.
JOURNAL EMBO J. 1990 Aug;9(8):2459-64.
PUBMED 2369898
REFERENCE 2 (bases 1 to 5385)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5385
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(63..80)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(234..822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(314..333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(993..1853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1616..1635
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1854..1958)
/gene="bla"
/label=AmpR promoter
rep_origin 1985..2440
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2072..2091)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2282..2303
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2566..2588
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2580..2597
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2581..2597
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 2758..3137
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3138..3341
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3292..3312
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
polyA_signal 4166..4788
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(4365..4384)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(4812..4832)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4817..5146
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4997..5132
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5059..5078
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5184..5203)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5185..5203)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5217..5233)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5217..5233)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5230..5252)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5241..5257
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5265..5295)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5310..5331
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
1981 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
2101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
2161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
2221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2341 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2401 tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc
2461 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
2521 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
2581 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
2641 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
2701 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
3001 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
3061 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
3121 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
3241 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
3361 attcgagctc ggtacccggg gatcctctag atgaccatcc ttttccttac tatggttatt
3421 tcatacttcg gttgcatgaa ggcggcgccc atgaaagaag taaacgtcca cggacaaggc
3481 aacttggcct acccaggtgt gcggacccat gggactctgg agagcgtgaa tgggcccagg
3541 gcaggttcga gaggtctgac gacgacatca ctggctgaca cttttgagca cgtcatcgaa
3601 gagctgctgg atgaggacca gaaggttcgg cccaacgaag aaaaccataa ggacgcggac
3661 ttgtacactt cccgggtgat gctcagcagt caagtgcctt tggagcctcc tctactcttt
3721 ctgctggagg aatacaaaaa ttacctggat gccgcaaaca tgtctatgag ggttcggcgc
3781 cactccgacc ctgcccgccg tggggagctg agcgtgtgtg acagtattag cgagtgggtc
3841 acagcggcag ataaaaagac tgcagtggac atgtctggcg ggacggtcac agtcctagag
3901 aaagtcccgg tatccaaagg ccaactgaag cagtatttct acgagaccaa gtgtaatccc
3961 atgggttaca ccaaggaagg ctgcaggggc atagacaaaa ggcactggaa ctcgcaatgc
4021 cgaactaccc aatcgtatgt tcgggccctt actatggata gcaaaaagag aattggctgg
4081 cgattcataa ggatagacac ttcctgtgta tgtacactga ccattaagag gggaagatag
4141 tctagagtcg actctagagg atcccgggtg gcatccctgt gacccctccc cagtgcctct
4201 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
4261 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
4321 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
4381 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
4441 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
4501 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
4561 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
4621 cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca
4681 gacacagcat aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg
4741 cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc
4801 agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
4921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
4981 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
5041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
5101 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga
5161 actgaaaaac cagaaagtta attccctata gtgagtcgta ttaaattcgt aatcatgtca
5221 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
5281 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
5341 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctg
//
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